•  
  •  
 

Abstract

Grape (Vitis vinifera L.) is one of the most economically significant fruit crops which has a large adaptive range, nutrition and medicine effect. A variety of 70 types of grapes are planted on more than 11.6 million plants in Iraq, generating a total annual output of more than 421,000 tons. Increasing grape quality, and particularly the phenolic composition recently has been of major interest. Leaf nutrition – particularly seaweed extracts, being rich in amino acids, trace elements and growth promoting substances – has proved to be a potential tool to enhance plant growth and fruit biochemical quality. The effect of grape variety and fertilizer on the phenolic content in grape fruits (Halwani and Kamali) was investigated using the foliar-applied seaweed extract, Agrimax® at 0, 15 and 30 ml/L were applied. Factorial experiment was carried out in 2024 with completely randomized design by three replications. The first foliar treatment was performed on the first day of April and thereafter five sprays were given at 15 days intervals. HPLC was used to determine the amount of phenolic compounds, such as caffeic, gallic, cinnamic, chlorogenic and p-coumaric acid), The data was analyzed using GenStat with LSD at 5% and two-way ANOVA followed by Šidák test. High phenolic traits were found to be significantly different in all, but caffeic, gallic, and cinnamic acids where the Halwani population extracted with methanol outperformed Kamali. Application of seaweed extract (particularly at 30 ml/L) considerably raised contents of caffeic, gallic, cinnamic and chlorogenic acids in comparison to control plants. The highest levels of all significantly altered phenolics were observed in Halwani variety at the concentration 30 ml/L Agrimax®. The level of p-coumaric acid did not significantly change. In conclusion, the Halwani cultivar had the highest phenolic content under Babil Germplasm climatological conditions. Application of seaweed extract by foliar method particularly at 30 ml/L significantly increased phenolic content, indicating its potential to be used as an eco-friendly substitute to mineral fertilization.

References

[1] Hussain SZ, Naseer B, Qadri T, Fatima T, Bhat TA. Grapes (Vitis vinifera)―Morphology, taxonomy, composition and health benefits. In: Fruits grown in highland regions of the himalayas: nutritional and health benefits. Springer; 2021. p. 103—15.

[2] Khan N, Fahad S, Naushad M, Faisal S. Grape production critical review in the world. 2020. Available SSRN 3595842.

[3] Altamimi A, Bakr UT. Determining the optimal size of production and measuring the specialized efficiency in grape production farms in Diyala Governorate - Iraq for the season 2021. Tikrit J Agric Sci 2023;23(4):117—35. https:// doi.org/10.25130/tjas.23.4.11.

[4] Badhani B, Sharma N, Kakkar R. Gallic acid: a versatile antioxidant with promising therapeutic and industrial applications. RSC Adv 2015;5(35):27540—57.

[5] Khan F, Bamunuarachchi NI, Tabassum N, Kim Y-M. Caffeic acid and its derivatives: antimicrobial drugs toward microbial pathogens. J Agric Food Chem 2021;69(10): 2979—3004.

[6] Wang L, et al. The biological activity mechanism of chlorogenic acid and its applications in food industry: a review. Front Nutr 2022;9:943911.

[7] Sova M. Antioxidant and antimicrobial activities of cinnamic acid derivatives. Mini Rev Med Chem 2012;12(8): 749—67.

[8] Kaur J, Kaur R. p-Coumaric acid: a naturally occurring chemical with potential therapeutic applications. Curr Org Chem 2022;26(14):1333—49. AL-BAHIR (JOURNAL FOR ENGINEERING AND PURE SCIENCES) 2026;8:184—192 191

[9] Al-Taey DKA, abd Al-Ameer A. Effect of salinity on the growth and yield of grapes: a review. In: IOP conference series: earth and environmental science. IOP Publishing; 2023. p. 42038.

[10] Singh J, et al. A review on the nutritional value and health benefits of different parts of grape (Vitis vinifera L.). Trop J Nat Prod Res 2023;7(9).

[11] Farhan M. Cytotoxic activity of the red grape polyphenol resveratrol against human prostate cancer cells: a molecular mechanism mediated by mobilization of nuclear copper and generation of reactive oxygen species. Life 2024;14(5):611.

[12] Ren B, et al. Resveratrol for cancer therapy: challenges and future perspectives. Cancer Lett 2021;515:63—72.

[13] James A, et al. A review on the influence of fertilizers application on grape yield and quality in the tropics. J Plant Nutr 2023;46(12):2936—57.

[14] Singh J, et al. “An introduction of plant nutrients and foliar fertilization: a review,” Precis. Farming a new approach, New Delhi Daya Publ. Co.. 2013. p. 252—320.

[15] Niu J, Liu C, Huang M, Liu K, Yan D. Effects of foliar fertilization: a review of current status and future perspectives. J Soil Sci Plant Nutr 2021;21(1):104—18.

[16] Aly A, Eliwa N, Abd El Megid MH. Improvement of growth, productivity and some chemical properties of hot pepper by foliar application of amino acids and yeast extract. Slovak J Food Sci 2019;13(1).

[17] Yüksel B, Oncü € T, S¸ en N. Assessing caffeine levels in soft beverages available in Istanbul, Turkey: an LC-MS/MS application in food toxicology. Toxicol Anal Clin 2023;35(1): 33—43.

[18] Younos MA, Akl EM. Evaluation of phytochemical analysis for chia (Salvia hispanica L.) and papaya (Carica papaya L.) seed extracts as antioxidant, antifungal and anti-aflatoxins. Egypt J Chem 2024;67(10):545—57. https://doi.org/10.21608/ ejchem.2024.270919.9346.

[19] Akram MT, et al. Comparative assessment of bioactive compounds, fruit quality attributes and sugar profiling in early maturing table grape (Vitis Vinifera L.) cultivars from Pothohar, Pakistan. Appl Fruit Sci 2024;66(3):983—95.

[20] Khan J, et al. Overview of the composition of whole grains' phenolic acids and dietary fibre and their effect on chronic non-communicable diseases. Int J Environ Res Publ Health 2022;19(5):3042.

[21] Hassoon AS, Almyali AAH, Kadhim AA, Jader JJ. Role of nano organic fertilizer in improving content of rocket (Eruca sativa mill) varieties from some secondary metabolism compounds. Lat Am J Pharm 2023;1(42):50—4.

[22] Mahdi AN, Abdullah KM, Almyali AAH. Effect of spraying with amino acids (tryptophan and glycine) and nano-potassium on some vegetative growth characteristics of stevia plant (Stevia rebaudiana Bertoni). In: IOP conference series: earth and environmental science. IOP Publishing; 2023. p. 102004.

[23] Garrido A, Engel J, Mumm R, Conde A, Cunha A, De Vos RCH. Metabolomics of photosynthetically active tissues in white grapes: effects of light microclimate and stress mitigation strategies. Metabolites 2021;11(4):205.

[24] Maia MRG. Metabolic characterization of grapevine leaves: first clues towards biomarkers discovery [PhD thesis]. University of Lisbon, Lisbon 2016.

[25] Liu Q, et al. Comparison of antioxidant activities of different grape varieties. Molecules 2018;23(10):2432.

[26] Yang S, et al. Effects of seaweed-extract-based organic fertilizers on the levels of mineral elements, sugar—acid components and hormones in Fuji apples. Agronomy 2023;13(4): 969.

[27] Casanova-Saez  R, Mateo-Bonmatí E, Ljung K. Auxin metabolism in plants. Cold Spring Harbor Perspect Biol 2021; 13(3):a039867.

[28] Teixeira A, Eiras-Dias J, Castellarin SD, Geros H. Berry phenolics of grapevine under challenging environments. Int J Mol Sci 2013;14(9):18711—39.

[29] Hussain MH, Hassoon AS. Measurement of some medical substances in Rosella plant (Hibiscus sabdariffa L.) extract using HPLC technique under the influence of different treatments. 2018.

[30] Pei K, Ou J, Huang J, Ou S. p-Coumaric acid and its conjugates: dietary sources, pharmacokinetic properties and biological activities. J Sci Food Agric 2016;96(9):2952—62.

[31] Kumawat P, Kumawat V. Seaweed marine algae: nutritional values and plant growth regulators for sustainable agriculture. Int J Environ Agric Res 2023;9.

[32] Hudecek M, Nozkova V, Plíhalova L, Plíhal O. Plant hormone cytokinin at the crossroads of stress priming and control of photosynthesis. Front Plant Sci 2023;13:1103088.

[33] Ninkuu V, et al. Phenylpropanoids metabolism: recent insight into stress tolerance and plant development cues. Front Plant Sci 2025;16:1571825.

[34] Lam VP, Bok G, Loi DN, Do MC, Park J. Seaweed foliar biostimulants improve growth and phytochemicals of Thai Basil (Ocimum basilicum L.) in a Plant Factory. Plants 2025; 14(21):3271.

[35] Ferhan M. Investigation of biological process for the conversion of bark biomass to bio-based polyphenols. 2016.

[36] Cao Y, Chen Y, Zhang L, Cai Y. Two monolignoid biosynthetic genes 4-coumarate: coenzyme A ligase (4CL) and pcoumaric acid 3-hdroxylase (C3H) involved in lignin accumulation in pear fruits. Physiol Mol Biol Plants 2023;29(6): 791—8.

[37] Marchiosi R, et al. Biosynthesis and metabolic actions of simple phenolic acids in plants. Phytochem Rev 2020;19(4): 865—906.

Share

COinS