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Al-Bahir Journal for Engineering and Pure Sciences

Abstract

Background: Ipomoea cairica (L.) sweet Convolvulaceae also known as morning glory or ‘Railway creeper’ is a traditional herb that has been used in treating various diseases throughout the world. Some of the reported pharmacological activities include antioxidant, anti-inflammatory, and antiviral. Cadmium is a toxic metal that can cause both hearts and testicular damage and its major mechanism is oxidative stress. Objective: Therefore, this study was done to investigate the effect of I. cairica leaf extract (ICE) against cadmium chloride-induced hearts and testicular damage in Wistar rats. Materials and Methods: Twenty male Wistar rats were divided into 4 groups of 5 animals each. Group I: Control (administered distilled water), Group II (administered 35 mg/kg CdCl2 intraperitoneally), group III (orally administered 100 mg/kg ICE and CdCl2), group IV (orally administered 100 mg/kg, I. cairica and CdCl2). Animals were sacrificed via cervical dislocation 24 hours after the last administration. The hearts and testes were collected and processed for biochemical assays. Biochemical assays evaluated were Malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and Glutathione-S-transferase (GST). Results: Results showed that Cd exposure significantly increases the level of MDA, decreased the concentration of GSH (non-enzymatic antioxidant), and decreased the activity of CAT, SOD, and GST (enzymatic antioxidants). Treatment with ICE prevented the alteration of redox status by CdCl2, as observed in the significant decrease in MDA concentration, and increase in GSH concentration when compared with the animals in the untreated groups. In addition, treatment with I. cairica also caused a significant increase in the activity of CAT, SOD, and GST as compared to the untreated group (P

References

  1. Rafati K, Rahimzadeh M, Rafati RM, Moghadamnia A. Arsenic compounds toxicity. Med Sci 2013;15:51e68. https://www.ncbi.nlm.nih.gov/pubmed/23984071
  2. Cuypers A, Plusquin M, Remans T, Jozefczak M, Keunen E, Gielen H, et al. Cadmium stress: an oxidative challenge. Biometals 2010;23:927e40. https://www.ncbi.nlm.nih.gov/pubmed/20697733
  3. Matovi CV, Buha A, Đukic-Cosi C, Bulat DZ. Insight into the oxidative stress induced by lead and or cadmium in blood,liver and kidneys. Food Chemistry Toxicology 2015;78:130e40. https://www.ncbi.nlm.nih.gov/pubmed/25660255
  4. Pizent A, Tariba B, Zivkovic T. Reproductive toxicity of metals in men. Arh Hig Rada Toksikol 2012;63:35e46. https://www.ncbi.nlm.nih.gov/pubmed/22462498
  5. Manfo FP,Moundipa PF,DeuchaudH, TchanaAN, Nantia EA, Zabot MT, et al. Effect of agropesticides use on a male reproductive function. Environ Toxicol 2012;27(7):423e32. https://www.ncbi.nlm.nih.gov/pubmed/20824615
  6. Wang J, Zhu H, Wang K, Yang Z, Liu Z. Protective effect of quercetin on rat testes against cadmium toxicity by alleviating oxidative stress and autophagy. Environ Sci Pollut Res Int 2020;27(20):25278e86. https://www.ncbi.nlm.nih.gov/pubmed/32488770
  7. Yari A, Sarveazad E, Asad J, Raouf SA, Babahajian N, Amini F, et al. Efficacy of Crocus Stativus L. on reduction of cadmium-Induced toxicity on spermatogenesis in adult rat. Andrologia 2016;48(10). https://www.ncbi.nlm.nih.gov/pubmed/26661047
  8. Shumaila S, Muhammad AH, Rafia R, Muhammad R. Garlic. In: Hanif MA, Nawaz H, Khan MM, Byrne HJ, editors. Medicinal plants of South Asia. Amsterdam, The Netherlands: Academic Press; Elsevier; 2020. p. 301e15.
  9. Kishore S, Anitha K, Shireesha N, Prathima K, Ravikumar A. A review on Ipomoea palmate. J Global Trends Pharmaceut Sci 2014;5(4):2151e3.
  10. Ilesanmi OB, Odewale TT, Avwioroko OJ, Alqarni M, Obadidullah AJ, Atanu FO, et al. Neuroprotective activity of Ipomoea cairica leaf extract against cadmium chloride induced biochemical changes in the brain of male Wistar rats. Bull Natl Res Cent 2022;46(1):1e10. https://doi.org/10.1186/s42269-021-00630-4
  11. Ilesanmi OB, Inala EI. Hepatoprotective effect of Ipomoea cairica (Con-volvulaceae) leaf extract against cadmium chloride induced liver damage. Toxicologie Analytique & Clinique; 2022. https://doi.org/10.1016/j.toxac.2021.12.001.
  12. Varshney R, Kale RK. Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes. Int J Radiat Biol 1990;58:733e43.
  13. Jollow DJ, Mitchel JR, Potter WZ, Davis DC, Gillette JR,Brodie BB. Acetaminophen-induced hepatic necrosis. II. Role of covalent binding in vivo. J Pharmacol Exp Therapeut 1973;187:195e202.
  14. Aebi H. Methods of enzymatic analysis. ed), 2 1974.p. 673e84.
  15. Misra HP, Fridovich I. The univalent reduction of oxygen by reduced flavins and quinones. J Biol Chem 1972;247:188e92.
  16. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases: the first enzymatic step in mercapturic acid formation.J Biol Chem 1974;249:7130e9.
  17. Chen YY, Zhu Y, Chan KM. Effects of cadmium on cell proliferation, apoptosis, and proto-oncogene expression in zebrafish liver cells.Aquat Toxicol 2014;157:196e206.
  18. ElmallahMI,ElkhadagryMF,Al-OlayanEM,AbdelMoneimAE.Protective effect of Fragaria ananassacrude extract on cadmiuminduced lipid peroxidation, antioxidant enzymes suppression,and apoptosis in rat testes.Int J Mol Sci 2017;18(5):957.https://doi.org/10.3390/ijms18050957
  19. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39(1):44e84.
  20. Tandon SK, Singh S, Prasad S, Khandekar K, Dwivedi VK, Chatterjee M, et al. Reversal of cadmium induced oxidative stress by chelating agent, antioxidant or their combination in rat. Toxicology 2003;145:211e7.
  21. Im JY, Paik SG, Han PL. Cadmium-induced astrological death proceeds via glutathione depletion. Neurosis 2006;83:301e8.
  22. Sarkar S, Yadav P, Trivedi R, Bansal AK, Bhatnagar D. cadmium-induced lipid peroxidation and the status of the antioxidant system in rat tissues. J Trace Elem Med Biol 1995;9(3):14e149.
  23. Li RX, Luo L. The protective effects of melatonin against oxidative stress and inflammation induced by acute cadmium exposure in mice testes. Biol Trace Elem Res 2016;170(1):152e64.
  24. Zhang Q, Zhang C, Ge J, Lliov MW, Talkuder M, Guo K, et al. Ameliorative effects of resveratrol against cadmium-induced nephrotoxicity via modulating nuclear xenobiotic receptors response and PINK1/Parkin-mediated mycophagy. Food 2020;11:1856e68.
  25. Amanpour P, Khodarahmi P, Salehipour M. Protective effect of Vitamin E on cadmium-induced apoptosis in rat testes. Naunyn-Shmiedeberg’s Archives of Pharmacology 2020;393(3):349e58.
  26. Casalino E, Sblano C, Landriscina V, Calzaretti G, Landriscina C. Rat liver glutathione S-transferase activity stimulation following acute cadmium or manganese intoxication. Toxicology 2004;200(1):29e38.
  27. Sahu RK, Kar M, Routray R. DPPH free radical scavenging activity of some leafy vegetables used by tribal of Odisha. India J Med Plan 2013;1(4):21e7.
  28. Ferrira AA, Amaral FA, G Duarte ID, Oliveira PM, Alves RB, Silveira D, et al. Antinociceptive effect from Ipomoea cairica extract. J Ethnopharmacol 2006;105(1e2):148e53.

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